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| Lafont, Maxime; Vergnes, Agnes; Vidal-dupiol, Jeremie; De Lorgeril, Julien; Gueguen, Yannick; Haffner, Philippe; Petton, Bruno; Chaparro, Cristian; Barrachina, Celia; Destoumieux-garzon, Delphine; Mitta, Guillaume; Gourbal, Benjamin; Montagnani, Caroline. |
| Over the last decade, innate immune priming has been evidenced in many invertebrate phyla. If mechanistic models have been proposed, molecular studies aiming to substantiate these models have remained scarce. We reveal here the transcriptional signature associated with immune priming in the oyster Crassostrea gigas. Oysters were fully protected against Ostreid herpesvirus 1 (OsHV-1), a major oyster pathogen, after priming with poly(I·C), which mimics viral double-stranded RNA. Global analysis through RNA sequencing of oyster and viral genes after immune priming and viral infection revealed that poly(I·C) induces a strong antiviral response that impairs OsHV-1 replication. Protection is based on a sustained upregulation of immune genes, notably genes... |
| Tipo: Text |
Palavras-chave: Innate immunity; Priming; OsHV-1; Antiviral response; Immune memory; Oyster; POMS; Poly(I·C); Interferon; Transcriptomic. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00614/72580/71541.pdf |
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| De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume. |
| Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial... |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf |
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| Lafont, Maxime; Petton, Bruno; Vergnes, Agnes; Pauletto, Marianna; Segarra, Amelie; Gourbal, Benjamin; Montagnani, Caroline. |
| In the last decade, a paradigm shift has emerged in comparative immunology. Invertebrates can no longer be considered to be devoid of specific recognition and immune memory. However, we still lack a comprehensive view of these phenomena and their molecular mechanisms across phyla, especially in terms of duration, specificity, and efficiency in a natural context. In this study, we focused on a Lophotrochozoan/virus interaction, as antiviral priming is mostly overlooked in molluscs. Juvenile Crassostrea gigas oysters experience reoccurring mass mortalities events from Ostreid herpes virus 1 with no existing therapeutic treatment. Our results showed that various nucleic acid injections can prime oysters to trigger an antiviral state ultimately protecting them... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00407/51811/52413.pdf |
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| Younes, Wan; Bensoussan, N; Romano, Jc; Arlhac, D; Lafont, Maxime. |
| Between 1996 and 2000, 79 cruises have been achieved following always the same transect between Marseilles and the Rhone river's mouth. While cruising, salinity, temperature and fluorescence of surface waters (1.5 m) were recorded every minute, in association with precise GPS position. The whole set of data. (i.e. 128 measured points per cruise) constitutes the Sorcom series whose synthetic results ate presented here. Despite the high spatial and temporal. variability encountered between two successive cruises, the regularity and duration of the sampling strategy permitted to define coherent spatial zonation and temporal evolutions. In the Cape Couronne vicinity the dynamic of the eastern extension of the dilution plume clearly changes. Westward, waters... |
| Tipo: Text |
Palavras-chave: Rhône; Panache de dilution; Observation à long terme; Fluorescence; Salinité; Rhone river; Dilution plume; Long term observation; Fluorescence; Salinity. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00321/43260/42991.pdf |
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